Journal: Stem Cells International

Article Title: Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cells

doi: 10.1155/2022/1092184

Figure Lengend Snippet: Nfic modulates pluripotency gene expression in mesenchymal stem cells of mineralized tissues. (a) Lin28 and Nanog gene expression levels were analyzed in control, Nfic -overexpressing, and (b) Sox2-overexpressing DPSC. (c) Lin28 and Nanog gene expression levels were analyzed in control, Nfic -overexpressing, and (d) Sox2-overexpressing BMSC. (e) (A–D) The number of ALP-positive colonies of iPSC cultured in OSKM medium compared to the addition of Nfic . (E–H) The number of ALP-positive colonies of iPSC cultured in OKM medium compared to the addition of Nfic . Scale bars = (A), (C), (E), (G) = 200 μ m. (f) Quantitative analysis of the number of ALP-positive colonies in various conditioned media. Data are presented as the mean ± SD. Abbreviations: ALP: alkaline phosphatase; BMSC: bone marrow stem cell; Ctrl: control; DPSC: dental pulp stem cell; iPSC: induced pluripotent stem cell; OSKM, Oct4, Sox2, Klf4, c-Myc; OKM, Oct4, Klf4, c-Myc.

Article Snippet: Briefly, pMX-retroviral vectors coding for human Oct4, Sox2, Klf4, and c-Myc (Addgene, Watertown, MA), Nfic, and packaging vectors pCMV-VSVG were co-transfected into GP2-293 cells using calcium-phosphate mammalian transfection kit (Takara Bio USA, Inc., San Jose, CA).

Techniques: Expressing, Cell Culture

Journal: Stem Cells International

Article Title: Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cells

doi: 10.1155/2022/1092184

Figure Lengend Snippet: Nfic does not affect Sox2 expression in soft tissues. (a) Sox2 mRNA and (b) protein expression levels were analyzed in primary cultured fibroblasts harvested from the tongues of Nfic −/− mice. (c) Immunohistochemistry (IHC) analysis of Sox2 expression in the tongues. (B) and (D) are higher magnifications of boxed (A) and (C). 6 weeks. Scale bars = (A), (C) = 50 μ m; (B), (D) = 20 μ m. (d) Proliferation rate of tail, (e) gingival, and (f) dermal fibroblasts from Nfic −/− mice from day 0 to 5 compared to the WT. n = 5, ∗ p < .05. (g) The number of ALP-positive colonies of iPSC from fibroblasts cultured in OSKM medium compared to the addition of Nfic in a dose-dependent manner. (A), (C), and (E) are higher magnifications of (B), (D), and (F), respectively. (h) Quantitative analysis of the number of ALP-positive colonies in various conditioned media. Data are presented as the mean ± SD. Abbreviations: ALP: alkaline phosphatase; Epi: epithelium; IHC: immunohistochemistry; iPSC: induced pluripotent stem cell; LaPr: lamina propria; OSKM, Oct4, Sox2, Klf4, c-Myc; OKM, Oct4, Klf4, c-Myc; SkMu, skeletal muscle; SqEp: squamous epithelium; WT: wild type.

Article Snippet: Briefly, pMX-retroviral vectors coding for human Oct4, Sox2, Klf4, and c-Myc (Addgene, Watertown, MA), Nfic, and packaging vectors pCMV-VSVG were co-transfected into GP2-293 cells using calcium-phosphate mammalian transfection kit (Takara Bio USA, Inc., San Jose, CA).

Techniques: Expressing, Cell Culture, Immunohistochemistry